goat anti mouse igg2b pe cy7  (SouthernBiotech)

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet: Antibody titration and validation All antibodies were titrated separately; here are a few examples to illustrate. We started with the manufacturer recommended dilution and made at least 6-point 1:2 dilution series plus unstained control. (A) WC1-IgG2a-FITC: recommended dilution 1:10 corresponds to 1 μg/mL. (B) CD45RO-IgG3-PE, the manufacturer does not provide the concentration corresponding to the recommended dilution 1:10. (C) CD25-IgG3: recommended dilution 1:100 corresponds to 10 μg/mL. (D) CD8-IgG1: recommended dilution 1:40 corresponds to 25 μg/mL. (E) IgG2a-APC-Cy7: recommended dilution 1:250 corresponds to 1 μg/mL. (F) IgG1-PE-Cy7: recommended dilution 1:40 corresponds to 5 μg/mL. Highlighted are the working dilutions that were defined for further use in the study.

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Titration, Biomarker Discovery, Control, Concentration Assay

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet: Staining procedure using an eight-step, seven-color assay

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Staining, Blocking Assay

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet:

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Virus, Recombinant, Sterility, Staining, Cell Culture, Hood, Flow Cytometry, Software

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet: Antibody titration and validation All antibodies were titrated separately; here are a few examples to illustrate. We started with the manufacturer recommended dilution and made at least 6-point 1:2 dilution series plus unstained control. (A) WC1-IgG2a-FITC: recommended dilution 1:10 corresponds to 1 μg/mL. (B) CD45RO-IgG3-PE, the manufacturer does not provide the concentration corresponding to the recommended dilution 1:10. (C) CD25-IgG3: recommended dilution 1:100 corresponds to 10 μg/mL. (D) CD8-IgG1: recommended dilution 1:40 corresponds to 25 μg/mL. (E) IgG2a-APC-Cy7: recommended dilution 1:250 corresponds to 1 μg/mL. (F) IgG1-PE-Cy7: recommended dilution 1:40 corresponds to 5 μg/mL. Highlighted are the working dilutions that were defined for further use in the study.

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Titration, Biomarker Discovery, Control, Concentration Assay

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet: Staining procedure using an eight-step, seven-color assay

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Staining, Blocking Assay

Journal: STAR Protocols

Article Title: Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

doi: 10.1016/j.xpro.2022.101688

Figure Lengend Snippet:

Article Snippet: Anti-mouse IgG2b, PE-Cy7 (Goat IgG; 1:250 dilution) , SouthernBiotech , Cat# 1090-17.

Techniques: Virus, Recombinant, Sterility, Staining, Cell Culture, Hood, Flow Cytometry, Software

Journal: International journal of cancer

Article Title: In vitro elimination of epidermal growth factor receptor-overexpressing cancer cells by CD32A-chimeric receptor T cells in combination with cetuximab or panitumumab

doi: 10.1002/ijc.32663

Figure Lengend Snippet: In vitro elimination of EGFR+ epithelial cancer cells (ECCs) by CD32A131R-chimeric receptor (CR) and CD16158F-CR T cells is associated with EGFR overexpression. (a) EGFR expression levels in the tumor cell lines as assessed by flow cytometry. The cells were incubated with 3 μg/ml of purified anti-EGFR antibody, washed and then stained with a phycoerythrin (PE)-conjugated antimouse IgG (gray filled histograms). The cells incubated with PE-conjugated antimouse IgG were used as a negative control (light gray filled histograms). Mean fluorescence intensity (MFI) is indicated for each cell line. (b) Spearman’s correlation between EGFR expression levels (MFI) in the HCT116, A549, MDA-MB-231 and MDA-MB-468 cell lines and the ECC elimination by CD16158F-CR T cells (left panel) and CD32A131R-CR T cells (right panel) in combination with cetuximab. A regression line is shown in black. Spearman’s rank correlation coefficient (r) was 0.6316 for CD16158F-CR and 0.533 for CD32A131R-CR. This is a cumulative analysis of five experiments separately performed: 116 = HCT116, 231 = MDA-MB-231, 468 = MDA-MB-468 and 549 = A549 cells.

Article Snippet: PE-conjugated goat antimouse IgG (1012-09) was purchased by Southern Biotech (Birmingham, AL).

Techniques: In Vitro, Over Expression, Expressing, Flow Cytometry, Incubation, Purification, Staining, Negative Control, Fluorescence